Molecular identification of biological samples using barcode sequencing (includes sequencing, submission to NCBI database, phylogeny tree construction)

Bacteria (16S rRNA)

Fungi (ITS)

Plant (matK or RBCL)

Animal (COI)

Cell culture and treatment

RNA Extraction

cDNA Synthesis (per three genes)

Gene expression analysis using real time PCR with   from  cDNA/gDNA and primers to be provided triplicate (To be planned based on discussion and background data)

SDS -PAGE

Agarose gel electrophoresis